Peptide Reconstitution Calculator
Important for NAD+ users: Please do not use this calculator for NAD+ vials. Those vials are 10 mL capacity — this tool is designed specifically for 3 mL vials only.

Peptide Reconstitution Calculator

1. What’s in your vial?

2. How much BAC water will you add?

Vial capacity: 3 mL (do not exceed 3 mL total liquid)
Recommended: 1–2.5 mL to leave headspace (~0.5 mL) for safe mixing.

3. What’s your dose per injection?

4. How often will you inject?

How to Reconstitute Your Peptide

Important: Always use bacteriostatic water (BAC water). Sterile technique is essential to avoid contamination.

  1. Remove the peptide vial from the refrigerator and allow it to reach room temperature for 15–20 minutes (prevents condensation inside the vial).
  2. Clean the vial top (and BAC water vial top) with an alcohol wipe using a circular motion from center outward. Let air dry completely.
  3. Use the calculator on the left to determine the appropriate amount of BAC water based on your desired concentration.
  4. Draw — mL of bacteriostatic water into a sterile insulin syringe, ensuring no air bubbles.
  5. Insert the needle into the peptide vial at a 45-degree angle, directing it against the glass wall (NOT directly into the powder).
  6. Slowly inject the BAC water drop by drop down the side of the vial — avoid direct stream to prevent foaming or protein degradation.
  7. Remove the needle and gently swirl the vial in a circular motion. NEVER shake vigorously — this can destroy the peptide structure.
  8. If any cloudiness appears, let it sit for 2–3 minutes, then swirl gently again until the solution is completely clear.
  9. The final solution should be clear and colorless. Any persistent cloudiness or particles may indicate degradation — do not use.
  10. Label the vial with the reconstitution date and concentration. Store immediately in the refrigerator at 2–8°C (36–46°F).
  11. Reconstituted peptides are typically stable for 8–12 weeks (or longer) when stored properly in the refrigerator at 2–8°C. Always inspect for cloudiness, particles, or odor before each use — discard if any signs of degradation appear.

Tip: For best results, keep the vial upright during mixing and avoid unnecessary agitation. If you’re unsure about any step, consult detailed research protocols.

Important for NAD+ users: Do not use this calculator for NAD+ vials (10 mL capacity). This tool is for 3 mL vials only.
For research/educational use only.

Peptide Storage Guidelines

  • Dry (lyophilized) peptides: Store at –20°C (freezer) for 1–2+ years stability. Refrigerator (2–8°C) is fine for shorter periods. Protect from light and moisture.
  • Reconstituted peptides: Keep refrigerated at 2–8°C. Do not freeze after reconstitution. Typical stability: 8–12 weeks or longer with proper handling.
  • Always: Label with reconstitution date & concentration, keep upright, minimize air exposure, use fresh sterile needles each time.
  • Discard if: Cloudiness/particles don’t clear, unusual color, bad odor, or signs of contamination appear.
  • Never: Expose to heat (hot car, window, radiator), leave in light for long periods, or use contaminated tools.

NAD+ Reconstitution Notes

This calculator is not designed for NAD+ vials (they are 10 mL capacity). If using NAD+, follow these adjusted steps instead:

  • Use the full 10 mL vial capacity when calculating BAC water (do not limit to 3 mL).
  • Many NAD+ protocols use 5–10 mL of bacteriostatic water for reconstitution (adjust based on desired concentration).
  • Follow the same sterile technique, slow injection down the vial wall, and gentle swirling.
  • Store reconstituted NAD+ at 2–8°C; stability is typically 4–8 weeks (or longer with proper handling) — always inspect before use.
  • Consider using a larger syringe (e.g., 3 mL or 5 mL) if drawing larger volumes.

For NAD+-specific dosing and stability, refer to your research protocol or supplier guidelines.